首页期刊导航|中草药（英文版）
期刊信息/Journal information
中草药（英文版）
中草药（英文版）

季刊

1674-6384

chm@tiprpress.com;bjchm@tiprpress.com

022-23006901

300193

天津市南开区鞍山西道308号

中草药（英文版）/Journal Chinese Herbal MedicinesCSTPCD
正式出版
收录年代

    Biosynthesis of natural products from medicinal plants:Challenges,progress and prospects

    Shilin Chen
    1-2页
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Consensus statement on research and application of Chinese herbal medicine derived extracellular vesicles-like particles(2023 edition)

    Qing ZhaoTong WangHongbin WangPeng Cao...
    3-12页
    查看更多>>摘要：To promote the development of extracellular vesicles of herbal medicine especially the establishment of standardization,led by the National Expert Committee on Research and Application of Chinese Herbal Vesicles,research experts in the field of herbal medicine and extracellular vesicles were invited nation-wide with the support of the Expert Committee on Research and Application of Chinese Herbal Vesicles,Professional Committee on Extracellular Vesicle Research and Application,Chinese Society of Research Hospitals and the Guangdong Engineering Research Center of Chinese Herbal Vesicles.Based on the col-lation of relevant literature,we have adopted the Delphi method,the consensus meeting method com-bined with the nominal group method to form a discussion draft of"Consensus statement on research and application of Chinese herbal medicine derived extracellular vesicles-like particles(2023)".The first draft was discussed in online and offline meetings on October 12,14,November 2,2022 and April and May 2023 on the current status of research,nomenclature,isolation methods,quality standards and research applications of extracellular vesicles of Chinese herbal medicines,and 13 consensus opinions were finally formed.At the Third Academic Conference on Research and Application of Chinese Herbal Vesicles,held on May 26,2023,Kewei Zhao,convenor of the consensus,presented and read the consensus to the experts of the Expert Committee on Research and Application of Chinese Herbal Vesicles.The con-sensus highlights the characteristics and advantages of Chinese medicine,inherits the essence,and keeps the righteousness and innovation,aiming to provide a reference for colleagues engaged in research and application of Chinese herbal vesicles at home and abroad,decode the mystery behind Chinese herbal vesicles together,establish a safe,effective and controllable accurate Chinese herbal vesicle prevention and treatment system,and build a bridge for Chinese medicine to the world.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Strategies on biosynthesis and production of bioactive compounds in medicinal plants

    Miaoxian GuoHaizhou LvHongyu ChenShuting Dong...
    13-26页
    查看更多>>摘要：Medicinal plants are a valuable source of essential medicines and herbal products for healthcare and dis-ease therapy.Compared with chemical synthesis and extraction,the biosynthesis of natural products is a very promising alternative for the successful conservation of medicinal plants,and its rapid development will greatly facilitate the conservation and sustainable utilization of medicinal plants.Here,we summa-rize the advances in strategies and methods concerning the biosynthesis and production of natural prod-ucts of medicinal plants.The strategies and methods mainly include genetic engineering,plant cell culture engineering,metabolic engineering,and synthetic biology based on multiple"OMICS"technolo-gies,with paradigms for the biosynthesis of terpenoids and alkaloids.We also highlight the biosynthetic approaches and discuss progress in the production of some valuable natural products,exemplifying com-pounds such as vindoline(alkaloid),artemisinin and paclitaxel(terpenoids),to illustrate the power of biotechnology in medicinal plants.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Gain deeper insights into traditional Chinese medicines using multidimensional chromatography combined with chemometric approaches

    Xinyue YangPingping ZengJin WenChuanlin Wang...
    27-41页
    查看更多>>摘要：Traditional Chinese medicines(TCMs)possess a rich historical background,unique theoretical frame-work,remarkable therapeutic efficacy,and abundant resources.However,the modernization and inter-nationalization of TCMs have faced significant obstacles due to their diverse ingredients and unknown mechanisms.To gain deeper insights into the phytochemicals and ensure the quality control of TCMs,there is an urgent need to enhance analytical techniques.Currently,two-dimensional(2D)chromatogra-phy,which incorporates two independent separation mechanisms,demonstrates superior separation capabilities compared to the traditional one-dimensional(1D)separation system when analyzing TCMs samples.Over the past decade,new techniques have been continuously developed to gain action-able insights from complex samples.This review presents the recent advancements in the application of multidimensional chromatography for the quality evaluation of TCMs,encompassing 2D-gas chromatog-raphy(GC),2D-liquid chromatography(LC),as well as emerging three-dimensional(3D)-GC,3D-LC,and their associated data-processing approaches.These studies highlight the promising potential of multidi-mensional chromatographic separation for future phytochemical analysis.Nevertheless,the increased separation capability has resulted in higher-order data sets and greater demands for data-processing tools.Considering that multidimensional chromatography is still a relatively nascent research field,fur-ther hardware enhancements and the implementation of chemometric methods are necessary to foster its robust development.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Potential efficacy and mechanism of eight mild-natured and bitter-flavored TCMs based on gut microbiota:A review

    Wenquan SuYanan YangXiaohui ZhaoJiale Cheng...
    42-55页
    查看更多>>摘要：The mild-natured and bitter-flavored traditional Chinese medicines(MB-TCMs)are an important class of TCMs that have been widely used in clinical practice and recognized as safe long-term treatments for chronic diseases.However,as an important class of TCMs,the panorama of pharmacological effects and the mechanisms of MB-TCMs have not been systemically reviewed.Compelling studies have shown that gut microbiota can mediate the therapeutic activity of TCMs and help to elucidate the core principles of TCM medicinal theory.In this systematic review,we found that MB-TCMs commonly participated in the modulation of metabolic syndrome,intestinal inflammation,nervous system disease and cardiovas-cular system disease in association with promoting the growth of beneficial bacteria Bacteroides,Akkermansia,Lactobacillus,Bifidobacterium,Roseburia as well as inhibiting the proliferation of harmful bacteria Helicobacter,Enterococcus,Desulfovibrio and Escherichia-Shigella.These alterations,correspond-ingly,enhance the generation of protective metabolites,mainly including short-chain fatty acids(SCFAs),bile acid(BAs),5-hydroxytryptamine(5-HT),indole and gamma-aminobutyric acid(GABA),and inhibit the generation of harmful metabolites,such as proinflammatory factors trimethylamine oxide(TAMO)and lipopolysaccharide(LPS),to further exert multiplicative effects for the maintenance of human health through several different signaling pathways.Altogether,this present review has attempted to comprehensively summarize the relationship between MB-TCMs and gut microbiota by establishing the TCMs-gut microbiota-metabolite-signaling pathway-diseases axis,which may provide new insight into the study of TCM medicinal theories and their clinical applications.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Hypoxia inducible factor-1α related mechanism and TCM intervention in process of early fracture healing

    Wenxian ZhangFusen YangQikai YanJiahui Li...
    56-69页
    查看更多>>摘要：As a common clinical disease,fracture is often accompanied by pain,swelling,bleeding as well as other symptoms and has a high disability rate,even threatening life,seriously endangering patients'physical and psychological health and quality of life.Medical practitioners take many strategies for the treatment of fracture healing,including Traditional Chinese Medicine(TCM).In the early stage of fracture healing,the local fracture is often in a state of hypoxia,accompanied by the expression of hypoxia inducible factor-1α(HIF-1α),which is beneficial to wound healing.Through literature mining,we thought that hypoxia,HIF-1α and downstream factors affected the mechanism of fracture healing,as well as domi-nated this process.Therefore,we reviewed the local characteristics and related signaling pathways involved in the fracture healing process and summarized the intervention of TCM on these mechanisms,in order to inspirit the new strategy for fracture healing,as well as elaborate on the possible principles of TCM in treating fractures based on the HIF molecular mechanism.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Chemical composition and pharmacological activity of seco-prezizaane-type sesquiterpenes

    Ye JinYanqing XiePeng ZhangAfsar Khan...
    70-81页
    查看更多>>摘要：The seco-prezizaane-type sesquiterpenes(SPS),as a special class of sesquiterpenes with a highly oxida-tive five-ring cage structure and seven consecutive chiral centers,are isolated from the genus Illicium,which have a variety of biological activities,including neurotoxicity and neurotrophic effects,etc.This review summarizes the chemical constituents and pharmacological effects of SPS,and discusses the potential trend and scope of future research.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Chuanxiong Rhizoma extracts prevent liver fibrosis via targeting CTCF-c-MYC-H19 pathway

    Yajing LiFanghong LiMingning DingZhi Ma...
    82-93页
    查看更多>>摘要：Objective:Hepatic fibrosis has been widely considered as a conjoint consequence of almost all chronic liver diseases.Chuanxiong Rhizoma(Chuanxiong in Chinese,CX)is a traditional Chinese herbal product to prevent cerebrovascular,gynecologic and hepatic diseases.Our previous study found that CX extracts significantly reduced collagen contraction force of hepatic stellate cells(HSCs).Here,this study aimed to compare the protection of different CX extracts on bile duct ligation(BDL)-induced liver fibrosis and investigate plausible underlying mechanisms.Methods:The active compounds of CX extracts were identified by high performance liquid chromatogra-phy(HPLC).Network pharmacology was used to determine potential targets of CX against hepatic fibro-sis.Bile duct hyperplasia and liver fibrosis were evaluated by serologic testing and histopathological evaluation.The expression of targets of interest was determined by quantitative real-time PCR(qPCR)and Western blot.Results:Different CX extracts were identified by tetramethylpyrazine,ferulic acid and senkyunolide A.Based on the network pharmacological analysis,42 overlap targets were obtained via merging the can-didates targets of CX and liver fibrosis.Different aqueous,alkaloid and phthalide extracts of CX(CXAE,CXAL and CXPHL)significantly inhibited diffuse severe bile duct hyperplasia and thus suppressed hepatic fibrosis by decreasing CCCTC binding factor(CTCF)-c-MYC-long non-coding RNA H19(H1 9)pathway in the BDL-induced mouse model.Meanwhile,CX extracts,especially CXAL and CXPHL also suppressed CTCF-c-MYC-H19 pathway and inhibited ductular reaction in cholangiocytes stimulated with tauro-cholate acid(TCA),lithocholic acid(LCA)and transforming growth factor beta(TGF-β),as illustrated by decreased bile duct proliferation markers.Conclusion:Our data supported that different CX extracts,especially CXAL and CXPHL significantly allevi-ated hepatic fibrosis and bile duct hyperplasia via inhibiting CTCF-c-MYC-H19 pathway,providing novel insights into the anti-fibrotic mechanism of CX.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Magnolol and 5-fluorouracil synergy inhibition of metastasis of cervical cancer cells by targeting PI3K/AKT/mTOR and EMT pathways

    Yuanyuan ChenShanshan ChenKaiting ChenLanfang Ji...
    94-105页
    查看更多>>摘要：Objective:This study is designed to investigate the mode of action of the synergistic effect of 5-fluorouracil(5-FU)and magnolol against cervical cancer.Methods:Network pharmacological approach was applied to predict the molecular mechanism of 5-FU combined with magnolol against cervical cancer.CCK-8 assay,colony formation assay,immunofluores-cence staining,adhesion assay,wound healing mobility assay,cell migration and invasion assay and Western blot analysis were conducted to validate the results of in silico study.Results:Phosphatidylinositol 3 kinase(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(mTOR)signaling pathway was identified as the key pathway in silico study.The experimental results showed that 5-FU combined with magnolol strongly inhibited cervical cancer cell proliferation,induced the morphological change of HeLa cells by down-regulating the expression of α-actinin,tensin-2 and vin-culin.Moreover,magnolol enhanced inhibitory effect of 5-FU on the cell adhesion,migration and inva-sion.The phosphorylation of AKT and PI3K and the expression of mTOR were strongly inhibited by the combination of 5-FU and magnolol.Moreover,the expression of E-cadherin and β-catenin was upregu-lated and the expression of Snail,Slug and vimentin was down-regulated by the 5-FU together with mag-nolol.Conclusion:Taken together,this study suggests that 5-FU combined with magnolol exerts a synergistic anti-cervical cancer effect by regulating the PI3K/AKT/mTOR and epithelial-mesenchymal transition(EMT)signaling pathways.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Anemoside B4 inhibits SARS-CoV-2 replication in vitro and in vivo

    Mingyue XiaoRonghua LuoQinghua LiangHonglv Jiang...
    106-112页
    查看更多>>摘要：Objective:Anemoside B4(AB4),the most abundant triterpenoidal saponin isolated from Pulsatilla chinen-sis,inhibited influenza virus FM1 or Klebsiella pneumoniae-induced pneumonia.However,the anti-SARS-CoV-2 effect of AB4 has not been unraveled.Therefore,this study aimed to determine the antiviral activ-ity and potential mechanism of AB4 in inhibiting human coronavirus SARS-CoV-2 in vivo and in vitro.Methods:The cytotoxicity of AB4 was evaluated using the Cell Counting Kit-8(CCK8)assay.SARS-CoV-2 infected HEK293T,HPAEpiC,and Vero E6 cells were used for in vitro assays.The antiviral effect of AB4 in vivo was evaluated by SARS-CoV-2-infected hACE2-IRES-luc transgenic mouse model.Furthermore,label-free quantitative proteomics and bioinformatic analysis were performed to explore the potential antiviral mechanism of action of AB4.Type Ⅰ IFN signaling-associated proteins were assessed using Western blotting or immumohistochemical staining.Results:The data showed that AB4 reduced the propagation of SARS-CoV-2 along with the decreased Nucleocapsid protein(N),Spike protein(S),and 3C-like protease(3CLpro)in HEK293T cells.In vivo antivi-ral activity data revealed that AB4 inhibited viral replication and relieved pneumonia in a SARS-CoV-2 infected mouse model.We further disclosed that the antiviral activity of AB4 was associated with the enhanced interferon(IFN)-β response via the activation of retinoic acid-inducible gene I(RIG-1)like receptor(RLP)pathways.Additionally,label-free quantitative proteomic analyses discovered that 17 pro-teins were significantly altered by AB4 in the SARS-CoV-2 coronavirus infections cells.These proteins mainly clustered in RNA metabolism.Conclusion:Our results indicated that AB4 inhibited SARS-CoV-2 replication through the RLR pathways and moderated the RNA metabolism,suggesting that it would be a potential lead compound for the development of anti-SARS-CoV-2 drugs.
      原文链接:
    • NETL
    • NSTL
    • 万方数据