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中草药（英文版）
中草药（英文版）

季刊

1674-6384

chm@tiprpress.com;bjchm@tiprpress.com

022-23006901

300193

天津市南开区鞍山西道308号

中草药（英文版）/Journal Chinese Herbal MedicinesCSTPCD
正式出版
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    Jatonik polyherbal mixture induced rat liver MMPT pore opening in normal Wistar rat:In vitro and in vivo studies

    Olabinri P.FolashadeIbrahim Damilare BoyenleTolulope A.OyedejiFiyinfoluwa Demilade Ojeniyi...
    113-120页
    查看更多>>摘要：Objective:To assess acute toxicity,the in vitro and in vivo effects of methanol and ethyl acetate extracts(JME and J EE)of Jatonik polyherbal mixture on some mitochondria-related parameters and their effect on the activity of some liver enzymes.Methods:Acute toxicity of JME and JEE was determined using Lorke's method.In vitro and in vivo opening of the mitochondrial membrane permeability transition pore(MMPT pore)was spectrophotometrically assayed.Production of malondialdehyde(MDA)as an index of lipid peroxidation and the activity of mito-chondrial ATPase was evaluated in vitro and in vivo and the effect of JME and JEE on the activity of liver enzymes such as alkaline phosphatase(ALP),aspartate and alanine aminotransferase(AST and ALT)and gamma-glutamyl transferase(GGT)was also investigated.Results:JME had an LD50 of 3 808 mg/kg b.w whereas JEE had an LD50 greater than 5 000 mg/kg b.w.of rats.After the rats have been fed with both extracts,a photomicrograph of a piece of liver tissue showed no apparent symptoms of toxicity.From the in vitro and in vivo studies,both extracts prompted intact mitochondria to open their MMPT pores.When compared to the control,lipid peroxide product release and ATPase activity were significantly increased(P＜0.05)in vitro and in vivo.The activities of AST,ALT,and GGT were all reduced at 50 mg/kg when treated with JME,but the activity of AST was considerably enhanced when treated with JEE(P＜0.05).The results revealed that both JME and JEE of the Jatonik poly-herbal mixture had low toxicity,profound MMPTpore induction,and enhanced ATPase activity,but an increased MDA production.Conclusion:Jatonik extracts may be a promising target for drug development in diseases where there is dysregulation of apoptosis,however,further studies are needed to better clarify the molecular mecha-nism involved in these phenomena.
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    Effect of Rhei Radix et Rhizoma and Eupolyphaga Steleophaga on liver protection mechanism based on pharmacokinetics and metabonomics

    Gang FengJianli BiWenfang JinQi Wang...
    121-131页
    查看更多>>摘要：Objective:Based on metabonomics technology of high-performance liquid chromatography-mass spec-trometry(HPLC-MS/MS)and hydrogen nuclear magnetic resonance spectroscopy(1H NMR),the pharma-cokinetic characteristics and therapeutic mechanism of Rhei Radix et Rhizoma(RhRR,Dahuang in Chinese),Eupolyphaga Steleophaga(EuS,Tubiechong in Chinese)combined with RhRR acting on acute liver injury were explored.Methods:Models of acute liver injury were established,and the pharmacokinetic methods of five compo-nents of RhRR-EuS in rats were found by HPLC-MS/MS.The liver tissues of different groups of mice were analyzed by 1H NMR spectroscopy combined with multivariate statistical analysis to investigate the metabolomics of RhRR-EuS and RhRR.Results:Pharmacokinetic results showed there were different levels of bimodal phenomenon in different groups,and the absorption of free anthraquinone in RhRR increased after compatibility with EuS.In addi-tion,the pathological state of acute liver injury in rats can selectively promote the absorption of emodin,chrysophanol,physcion and aloe emodin.Through 15 differential metabolites in the liver tissue of acute liver injury mice,it was revealed that RhRR-EuS and RhRR could protect the liver injury by regulating the metabolism of glutamine and glutamic acid,alanine,aspartic acid and glutamic acid,and phosphoinosi-tide.However,the regulation of RhRR was weaker than that of RhRR-EuS.Conclusion:For the first time,we studied the pharmacokinetics and metabolomics differences of RhRR-EuS and RhRR in rats and mice with acute liver injury,in order to provide theoretical reference for clinical treatment of liver disease by DHZCP.
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    Effects of Xiaoyao San on exercise capacity and liver mitochondrial metabolomics in rat depression model

    Weidi ZhaoCui JiJie ZhengShi Zhou...
    132-142页
    查看更多>>摘要：Objective:This study aimed to investigate the therapeutic effects of Xiaoyao San(XYS),a herbal medicine formula,on exercise capacity and liver mitochondrial metabolomics in a rat model of depression induced by chronic unpredictable mild stress(CUMS).Methods:A total of 24 male SD rats were randomly divided into four groups:control group(C),CUMS control group(M),Venlafaxine positive treatment group(V),and XYS treatment group(X).Depressive behaviour and exercise capacity of rats were assessed by body weight,sugar-water preference test,open field test,pole test,and rotarod test.The liver mitochondria metabolomics were analyzed by using liquid chromatography-mass spectrometry(LC-MS)method.TCMSP database and GeneCards database were used to screen XYS for potential targets for depression,and GO and KEGG enrichment analyses were per-formed.Results:Compared with C group,rats in M group showed significantly lower body weight,sugar water preference rate,number of crossing and rearing in the open field test,climbing down time in the pole test,and retention time on the rotarod test(P＜0.01).The above behaviors and exercise capacity indices were significantly modulated in rats in V and X groups compared with M group(P＜0.05,0.01).Compared with C group,a total of 18 different metabolites were changed in the liver mitochondria of rats in M group.Nine different metabolites and six metabolic pathways were regulated in the liver mitochondria of rats in X group compared with M group.The results of network pharmacology showed that 88 intersecting targets for depression and XYS were obtained,among which 15 key targets such as IL-1β,IL-6,and TNF were predicted to be the main differential targets for the treatment of depression.Additionally,a total of 1 553 GO signaling pathways and 181 KEGG signaling pathways were identified,and the main biological pathways were AGE-RAGE signaling pathway,HIF-1 signaling pathway,and calcium signaling pathway.Conclusion:XYS treatment could improve depressive symptoms,enhance exercise capacity,positively regulate the changes of mitochondrial metabolites and improve energy metabolism in the liver of depressed rats.These findings suggest that XYS exerts antidepressant effects through multi-target and multi-pathway.
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    DNA metabarcoding analysis of fungal community on surface of four root herbs

    Yujie DaoJingsheng YuMeihua YangJianping Han...
    143-150页
    查看更多>>摘要：Objective:Angelicae Sinensis Radix(ASR,Danggui in Chinese),Cistanches Herba(CH,Roucongrong in Chinese),Ginseng Radix et Rhizoma(PG,Renshen in Chinese),and Panacis Quinquefolii Radix(PQ.Xiyangshen in Chinese),widely used as medicine and dietary supplement around the world,are suscep-tible to fungal and mycotoxin contamination.In this study,we aim to analyze their fungal community by DNA metabarcoding.Methods:A total of 12 root samples were collected from three main production areas in China.The sam-ples were divided into four groups based on herb species,including ASR,CH,PG,and PQ groups.The fun-gal community on the surface of four root groups was investigated through DNA metabarcoding via targeting the internal transcribed spacer 2 region(ITS2).Results:All the 12 samples were detected with fungal contamination.Rhizopus(13.04％-74.03％),Aspergillus(1.76％-23.92％),and Fusarium(0.26％-15.27％)were the predominant genera.Ten important fungi were identified at the species level,including two potential toxigenic fungi(Penicillium citrinum and P.oxalicum)and eight human pathogenic fungi(Alternaria infectoria,Candida sake,Hyphopichia bur-tonii,Malassezia globosa,M.restricta,Rhizopus arrhizus,Rhodotorula mucilaginosa,and Ochroconis tsha-wytschae).Fungal community in ASR and CH groups was significantly different from other groups,while fungal community in PG and PQ groups was relatively similar.Conclusion:DNA metabarcoding revealed the fungal community in four important root herbs.This study provided an important reference for preventing root herbs against fungal and mycotoxin contamination.
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    Hypoglycemic activities of flowers of Xanthoceras sorbifolia and identification of anti-oxidant components by off-line UPLC-QTOF-MS/MS-free radical scavenging detection

    Xiajing XuYongli GuoMenglin ChenNing Li...
    151-161页
    查看更多>>摘要：Objective:To identify phytochemical constituents present in the extract of flowers of Xanthoceras sorbifo-lia and evaluate their anti-oxidant and anti-hyperglycemic capacities.Methods:The AlCl3 colorimetric method and Prussian Blue assay were used to determine the contents of total flavonoids and total phenolic acids in extraction layers,and the bioactive layers was screened through anti-oxidative activity in vitro.The Waters ACQUITY UPLC system and a Waters ACQUITY UPLC BEH C18 column(2.0 mm × 150 mm,5 μm)were used to identify the ingredients.And anti-oxidative ingredients were screened by off-line UPLC-QTOF-MS/MS-free radical scavenging.The amelio-rative role of it was further evaluated in a high-fat,streptozotocin-induced type 2 diabetic rat model and the study was carried out on NADPH oxidase(PDB ID:2CDU)by molecular docking.Results:Combined with the results of activity screening in vitro,the anti-oxidative part was identified as the ethyl acetate layer.A total of 24 chemical constituents were identified by liquid chromatography-mass spectrometry in the ethyl acetate layer and 13 main anti-oxidative active constituents were prelim-inarily screened out through off-line UPLC-QTOF-MS/MS-free radical scavenging.In vivo experiments showed that flowers of X.sorbifolia could significantly reduce the blood glucose level of diabetic mice and alleviate liver cell damage.Based on the results of docking analysis related to the identified phyto-compounds and oxidase which involved in type 2 diabetes,quercetin 3-O-rutinoside,kaempferol-3-O-rhamnoside,isorhamnetin-3-O-glucoside,and isoquercitrin showed a better inhibitory profile.Conclusion:The ethyl acetate layer was rich in flavonoids and phenolic acids and had significant anti-oxidant activity,which could prevent hyperglycemia.This observed activity profile suggested X.sorbifolia flowers as a promising new source of tea to develop alternative natural anti-diabetic products with a high safety margin.
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    Chemical components in cultivated Cordyceps sinensis and their effects on fibrosis

    Zhonghua DongXiao Sun
    162-167页
    查看更多>>摘要：Objective:Cultivated Cordyceps sinensis powder has been used as clinical drug and healthy food to nour-ish the lung and kidney,which solves the problem of serious shortage of wild C.sinensis.This study aims to explore the chemical components and compared their anti-fibrotic effects in cultivated C.sinensis.Methods:Nucleosides,sterols and polysaccharides were separated and purified from cultivated C.sinen-sis,and analyzed by high performance liquid chromatography,gas chromatography-mass spectrometry and chemical chromogenic methods,respectively.In high glucose-induced rat mesangial cell models,fibronectin and type 1 collagen were used as evaluation indicators.Results:There were 10 kinds of nucleosides and one sterol in cultivated C.sinensis.The contents of nucle-osides,sterols and polysaccharides in the cultivated C.sinensis were close to 2％,0.55％and 4.4％,respec-tively.Furthermore,nucleoside,sterol and polysaccharide components exhibited varying degrees of anti-fibrotic activity.The nucleoside components and sterol components inhibited the expression of extracel-lular matrix more effectively in the three main components.Conclusion:Cultivated C.sinensis remains the similar compounds with the wild C.sinensis,and nucleo-sides and sterols may be the main active substances that contribute to its anti-fibrotic effects.The project of this study may provide valuable information on further optimization of more effective remedies with few side effects based on cultivated C.sinensis.
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    Ethical Guideline to Authors,Editors,and Reviewers

    后插5-后插6页
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    Instruction for Authors

    后插1-后插4页
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    The conference on the 40th anniversary of establishment of IMPLAD was held

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