期刊,中国科学:生命科学（英文版） 2024年卷11期_国家学术搜索

期刊信息/Journal information

中国科学:生命科学（英文版）

主　　编：周光召

出版周期：月刊

国际刊号：1674-7305

电子邮箱：sales@scichina.org

电　　话：010-64019820

邮政编码：100717

地　　址：北京东黄城根北街16号

中国科学:生命科学（英文版）/Journal Science China(Life Sciences)CSCDCSTPCDSCI

查看更多>>《中国科学》是中国科学院主办、中国科学杂志社出版的自然科学专业性学术刊物。《中国科学》任务是反映中国自然科学各学科中的最新科研成果，以促进国内外的学术交流。《中国科学》以论文形式报道中国基础研究和应用研究方面具有创造性的、高水平的和有重要意义的科研成果。在国际学术界，《中国科学》作为代表中国最高水平的学术刊物也受到高度重视。国际上最具有权威的检索刊物SCI，多年来一直收录《中国科学》的论文。1999年《中国科学》夺得国家期刊奖的第一名。

正式出版

收录年代

Unveiling potential sex-determining genes and sex-specific markers in autotetraploid Carassius auratus

Kun ZhangXu HuangChongqing WangXidan Xu...

2444-2458页

查看更多>>摘要：Autotetraploid Carassius auratus is a stable hereditary autotetraploid fish resulting from the hybridization of Carassius auratus red var.(RCC,♀)× Megalobrama amblycephala(BSB,♂),containing four sets of RCC chromosomes.However,the molecular mechanism underlying the determination of sex in this species remains largely unknown.Currently,there lacks a full understanding of the molecular mechanisms governing sex determination and specific molecular markers to differentiate sex in this species.In this study,25,801,677 SNPs(Single-nucleotide polymorphism)and 6,210,306 Indels(insertion-deletion)were obtained from whole-genome resequencing of 100 individuals(including 50 female and 50 male).Further identification confirmed the candidate chromosomes as Chr46B,with the sex-determining region located at Chr46B:22,500,000-22,800,000 bp.Based on the male-specific insertion(26 bp)within the candidate sex-determining region,a pair of sex-specific molecular markers has been identified.In addition,based on the screening of candidate sex-determining region genes and RT-qPCR validation analysis,ADAM10,AQP9 and tc1a were identified as candidate sex-determining genes.These findings provide a robust foundation for investigating sex determination mechanisms in fish,the evolution of sex chromosomes,and the development of monosex populations.

原文链接:

万方数据

Multi-omics analysis reveals the genetic and environmental factors in shaping the gut resistome of a keystone rodent species

Guoliang LiDong ZhuChaoyuan ChengHaiyan Chu...

2459-2470页

查看更多>>摘要：Understanding the emergence and spread of antibiotic resistance genes(ARGs)in wildlife is critical for the health of humans and animals from a"One Health"perspective.The gut microbiota serve as a reservoir for ARGs;however,it remains poorly understood how environ-mental and host genetic factors influence ARGs by affecting the gut microbiota.To elucidate this,we analyzed whole-genome resequencing data from 79 individuals of Brandt's vole in two geographic locations with different antibiotics usage,together with metabolomic data and shotgun sequencing data.A high diversity of ARGs(851 subtypes)was observed in vole's gut,with a large variation in ARG composition between individuals from Xilingol and Hulunbuir in China.The diversity and composition of ARGs were strongly correlated with variations in gut microbiota community structure.Genome-wide association studies revealed that 803 loci were significantly associated(P＜5.05x10-9)with 31 bacterial species,and bipartite networks identified 906 bacterial species-ARGs associations.Structural equation modeling analysis showed that host genetic factors,air temperature,and presence of pollutants(Bisphenol A)significantly affected gut microbiota community structure,which eventually regulated the diversity of ARGs.The present study advances our understanding of the complex host-environ-ment interactions that underlie the spread of ARGs in the natural environments.

原文链接:

万方数据

Versatile and efficient mammalian genome editing with Type Ⅰ-C CRISPR System of Desulfovibrio vulgaris

Pan LiDingcai DongFei GaoYuyang Xie...

2471-2487页

查看更多>>摘要：CRISPR-Cas tools for mammalian genome editing typically rely on single Cas9 or Cas12a proteins.While type Ⅰ CRISPR systems in Class Ⅰ may offer greater specificity and versatility,they are not well-developed for genome editing.Here,we present an alternative type Ⅰ-C CRISPR system from Desulfovibrio vulgaris(Dvu)for efficient and precise genome editing in mammalian cells and animals.We optimized the Dvu type Ⅰ-C editing complex to generate precise deletions at multiple loci in various cell lines and pig primary fibroblast cells using a paired PAM-in crRNA strategy.These edited pig cells can serve as donors for generating transgenic cloned piglets.The Dvu type Ⅰ-C editor also enabled precise large fragment replacements with homology-directed repair.Additionally,we adapted the Dvu-Cascade effector for cytosine and adenine base editing,developing Dvu-CBE and Dvu-ABE systems.These systems efficiently induced C-to-T and A-to-G substitutions in human genes without double-strand breaks.Off-target analysis confirmed the high specificity of the Dvu type Ⅰ-C editor.Our findings demonstrate the Dvu type Ⅰ-C editor's potential for diverse mammalian genome editing applications,including deletions,fragment re-placement,and base editing,with high efficiency and specificity for biomedicine and agriculture.

原文链接:

万方数据

6-Phosphogluconate dehydrogenase 2 bridges the OPP and shikimate pathways to enhance aromatic amino acid production in plants

Qian TangYuxin HuangZhuanglin ShenLinhui Sun...

2488-2498页

查看更多>>摘要：The oxidative pentose phosphate(OPP)pathway provides metabolic intermediates for the shikimate pathway and directs carbon flow to the biosynthesis of aromatic amino acids(AAAs),which serve as basic protein building blocks and precursors of numerous metabolites essential for plant growth.However,genetic evidence linking the two pathways is largely unclear.In this study,we identified 6-phosphogluconate dehydrogenase 2(PGD2),the rate-limiting enzyme of the cytosolic OPP pathway,through suppressor screening of arogenate dehydrogenase 2(adh2)in Arabidopsis.Our data indicated that a single amino acid substitution at position 63(glutamic acid to lysine)of PGD2 enhanced its enzyme activity by facilitating the dissociation of products from the active site of PGD2,thus increasing the accumulation of AAAs and partially restoring the defective phenotype of adh2.Phylogenetic analysis indicated that the point mutation occurred in a well-conserved amino acid residue.Plants with different amino acids at this conserved site of PGDs confer diverse catalytic activities,thus exhibiting distinct AAAs producing capability.These findings uncover the genetic link between the OPP pathway and AAAs biosynthesis through PGD2.The gain-of-function point mutation of PGD2 identified here could be considered as a potential engineering target to alter the metabolic flux for the production of AAAs and downstream compounds.

原文链接:

万方数据

Shifts in reproductive strategies in the evolutionary trajectory of plant lineages

Xin-Jian ZhangXian-Han HuangJacob B.LandisQuan-Sheng Fu...

2499-2510页

查看更多>>摘要：Understanding the maintenance and shift in reproductive strategies is a fundamental question in evolutionary research.Although many efforts have been made to compare different reproductive strategies,the association between reproductive strategies and lineage divergence is largely unknown.To explore the impact of different reproductive strategies on lineage divergence,we investigated the evolution of clonality in Saxifraga sect.Irregulares+Heterisia.By integrating several lines of evidence,we found that the loss of clonality in Irregulares+Heterisia was associated with a progressive increase in diversification rate and intraspecific morphological diversity but with a reduction in species distribution range.Our findings provide insights into the ecological and evolutionary effects of different reproductive strategies,suggesting the necessity of integrating clonality into ecological and evolutional research.

原文链接:

万方数据

MSC-mediated mitochondrial transfer restores mitochondrial DNA and function in neural progenitor cells of Leber's hereditary optic neuropathy

Rui WangFeixiang BaoManjiao LuXiaoyun Jia...

2511-2519页

查看更多>>摘要：Leber's hereditary optic neuropathy(LHON)is a debilitating mitochondrial disease associated with mutations in mitochondrial DNA(mtDNA).Unfortunately,the available treatment options for LHON patients are limited due to challenges in mitochondrial replacement.In our study,we reprogramming LHON urine cells into induced pluripotent stem cells(iPSCs)and differentiating them into neural progenitor cells(NPCs)and neurons for disease modeling.Our research revealed that LHON neurons exhibited significantly higher levels of mtDNA mutations and reduced mitochondrial function,confirming the disease phenotype.However,through co-culturing LHON iPSC-derived NPCs with mesenchymal stem cells(MSCs),we observed a remarkable rescue of mutant mtDNA and a significant improvement in mitochondrial metabolic function in LHON neurons.These findings suggest that co-culturing with MSCs can enhance mitochondrial function in LHON NPCs,even after their differentiation into neurons.This discovery holds promise as a potential therapeutic strategy for LHON patients.

原文链接: