Chloroplast genome of Phyllostachys edulis f.luteosulcata and comparison of chloroplast genome sequence of subspecies of Ph.edulis
[Objective]This study aims to sequence,assemble,annotate,and analyze the chloroplast genome of Phyllostachys edulis f.luteosulcata.The research also involves comparing its chloroplast genetic information and phylogenetic relationships with those of other subspecies of Ph.edulis(moso bamboo).[Method]High-throughput sequencing data were used to assemble and annotate the complete chloroplast genome of Ph.edulis f.luteosulcata.Subsequently,we analyze the composition,codon preference,and repetitive sequences of the genome.Furthermore,sequence comparison and phylogenetic analysis were conducted to compare the phylogenetic relationships and genome sequence differences among different subspecies of moso bamboo.[Result]The chloroplast genome of Ph.edulis f.luteosulcata is a double-loop DNA of 139 678 bp in length containing 132 genes,including 85 protein-coding genes,eight ribosomal RNAs(rRNAs),and 39 transfer RNAs(tRNAs).The codon preference for this genome has an A/U base at the end.There are 49 repetitive sequences with the most common type being A/T and 55 Simple Sequence Repeat(SSR)sites.Phylogenetic analyses constructed using the chloroplast genome sequences showed that Ph.edulis f.luteulosulcata is in a monophyletic branch together with other subspecies of Ph.edulis and is closely related to the original variety of Ph.edulis var.pubescens.The analysis of chloroplast genome sequence and coding gene characteristics showed that there were differences in the number and structure of coding genes and low degree of sequence variation among the subspecies of Ph.edulis.[Conclusion]This study is the first to comparatively analyze the chloroplast genomes of subspecies of Ph.edulis and reveals a degree of sequence variation in these subspecies of Ph.edulis.This variation information would be available for the identification and comparison of subspecies of Ph.edulis.[Ch,5 fig.2 tab.27 ref.]
国家科技期刊平台
