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畜牧与生物技术杂志(英文版)
中国科学技术协会
畜牧与生物技术杂志(英文版)

中国科学技术协会

李德发

季刊

1674-9782

zgxm@cau.edu.cn;xmzz@cau.edu.cn

010-62734403/2723

100193

中国农业大学西校区动物科技学院Journal of Animal Science and Biotechnology编辑部

畜牧与生物技术杂志(英文版)/Journal Journal of Animal Science and BiotechnologyCSCDCSTPCD北大核心SCI
查看更多>>定位为国内外公开发行的专业学术期刊，宗旨是立足国内，面向国际，为国内外畜牧科技工作者提供国际化学术共享的园地，促进我国与国际上其他国家在畜牧科学领域的交流；让中国畜牧科技走向世界，让世界了解中国畜牧科技。
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    Quercetin ameliorates oxidative stress-induced apoptosis of granulosa cells in dairy cow follicular cysts by activating autophagy via the SIRT1/ROS/AMPK signaling pathway

    Hongwei DuanFang WangKe WangShuai Yang...
    2351-2365页
    查看更多>>摘要：Background Follicular cysts contribute significantly to reproductive loss in high-yield dairy cows.This results from the death of follicular granulosa cells(GCs)caused by oxidative stress.Quercetin is known to have significant antioxidant and anti-apoptotic effects.However,the effect of quercetin on follicular cysts has yet been elucidated.Therefore,this study aimed to explore the anti-oxidant and anti-apoptosis effects and potential molecular mecha-nisms of quercetin in H2O2-induced primary cow GCs and 3-nitropropionic acid(3-NPA)-induced mouse model of oxi-dative stress and thus treat ovarian cysts in dairy cows.Results In this study,compared with estrus cows,cows with follicular cysts showed heightened levels of oxida-tive stress and increased follicular cell apoptosis,while autophagy levels were reduced.A model of oxidative stress was induced in vitro by H2O2 and showed significant increases in apoptosis together with reduced autophagy.These effects were significantly ameliorated by quercetin.Effects similar to those of quercetin were observed after treat-ment of cells with the reactive oxygen species(ROS)inhibitor N-acetylcysteine(NAC).Further investigations using chloroquine(autophagy inhibitor),rapamycin(autophagy activator),selisistat(SIRT1 inhibitor),and compound C(AMPK inhibitor)showed that chloroquine counteracted the effects of quercetin on oxidative stress-induced apop-tosis,while rapamycin had the same effect as quercetin.In addition,the SIRT1/AMPK pathway inhibitors antagonized quercetin-mediated mitigation of the effects of oxidative stress on increased apoptosis and reduced autophagy.Consistent with the results in vitro,in mouse ovarian oxidative stress model induced by 3-NPA,quercetin activated autophagy through the SIRT1/AMPK signaling pathway,while alleviating oxidative stress damage and inhibiting apoptosis in mouse ovaries.Conclusions These findings indicate that quercetin can inhibit apoptosis in GCs and restore ovarian function by acti-vating autophagy through the SIRT1/ROS/AMPK signaling pathway,suggesting a new direction for the treatment of ovarian follicular cysts in high-yield dairy cows.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Lipolysis pathways modulate lipid mediator release and endocannabinoid system signaling in dairy cows'adipocytes

    Madison N.MyersMiguel ChiriviJeff C.GandyJoseph Tam...
    2366-2382页
    查看更多>>摘要：Background As cows transition from pregnancy to lactation,free fatty acids(FFA)are mobilized from adipose tissues(AT)through lipolysis to counter energy defiicits.In clinically healthy cows,lipolysis intensity is reduced through-out lactation;however,if FFA release exceeds tissue demands or the liver's metabolic capacity,lipid byproducts accumulate,increasing cows'risk of metabolic and infectious disease.Endocannabinoids(eCBs)and their congeners,N-acylethanolamines(NAEs),are lipid-based compounds that modulate metabolism and inflammation.Their syn-thesis and release depend upon the availability of FFA precursors and the abundance of synthesizing and degrading enzymes and transporters.Therefore,we hypothesized that eCB production and transcription of endocannabinoid system components are modulated by lipolysis pathways in adipocytes.To test this hypothesis,we stimulated canoni-cal(isoproterenol,1 μmol/L;ISO)and inflammatory(lipopolysaccharide,1 μg/mL;LPS)lipolysis pathways in adipo-cytes isolated from the AT of 5 Holstein dairy cows.Following,we assessed lipolysis intensity,adipocytes'release of eCBs,and transcription of endocannabinoid system components.Results We found that ISO and LPS stimulated lipolysis at comparable intensities.Exposure to either treat-ment tended to elevate the release of eCBs and NAEs by cultured adipocytes;however,specific eCBs and NAEs and the transcriptional profiles differed by treatment.On one hand,ISO enhanced adipocytes'release of 2-ara-chidonoylglycerol(2-AG)but reduced NAE production.Notably,ISO enhanced the cells'expression of enzymes associated with 2-AG biosynthesis(INPP5F,GDPD5,GPAT4),transport(CD36),and adipogenesis(PPARG).Conversely,LPS enhanced adipocytes'synthesis and release of N-arachidonoylethanolamide(AEA).This change coincided with enhanced transcription of the NAE-biosynthesizing enzyme,PTPN22,and adipocytes'transcription of genes related to eCB degradation(PTGS2,MGLL,CYP27B1).Furthermore,LPS enhanced adipocytes'transcription of eCB and NAE transporters(HSPA1A,SCP2)and the expression of the anti-adipogenic ion channel,TRPV3.Conclusions Our data provide evidence for distinct modulatory roles of canonical and inflammatory lipolysis pathways over eCB release and transcriptional regulation of biosynthesis,degradation,transport,and ECS signaling in cows'adipocytes.Based on our findings,we conclude that,within adipocytes,eCB production and ECS compo-nent expression are,at least in part,mediated by lipolysis in a pathway-dependent manner.These findings contrib-ute to a deeper understanding of the molecular mechanisms underlying metabolic regulation in dairy cows'AT,with potential implications for prevention and treatment of inflammatory and metabolic disorders.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Melatonin alleviates palmitic acid-induced mitochondrial dysfunction by reducing oxidative stress and enhancing autophagy in bovine endometrial epithelial cells

    Yi WangJianfei GongNuo HengYingfan Hu...
    2383-2402页
    查看更多>>摘要：Background Negative energy balance(NEB)typically occurs in dairy cows after delivery.Cows with a high yield are more likely to experience significant NEB.This type of metabolic imbalance could cause ketosis,which is often accompanied by a decline in reproductive performance.However,the molecular mechanisms underlying NEB have yet to be fully elucidated.During excessive NEB,the body fat is extensively broken down,resulting in the abnormal accumulation of non-esterified fatty acids(NEFAs),represented by palmitic acid(PA),within the uterus.Such an abnor-mal accumulation has the potential to damage bovine endometrial epithelial cells(BEECs),while the molecular mechanisms underlying its involvement in the PA-induced injury of BEECs remains poorly understood.Melatonin(MT)is recognized for its regulatory role in maintaining the homeostasis of mitochondrial reactive oxygen species(mitoROS).However,little is known as to whether MT could ameliorate the damage incurred by BEECs in response to PA and the molecular mechanism involved.Results Analysis showed that 0.2 mmol/L PA stress increased the level of cellular and mitochondrial oxidative stress,as indicated by increased reactive oxygen species(ROS)level.In addition,we observed mitochondrial dys-function,including abnormal mitochondrial structure and respiratory function,along with a reduction in mito-chondrial membrane potential and mitochondrial copy number,and the induction of apoptosis.Notably,we also observed the upregulation of autophagy proteins(PINK,Parkin,LC3B and Ubiquitin),however,the P62 protein was also increased.As we expected,100 μmol/L of MT pre-treatment attenuated PA-induced mitochondrial ROS and restored mitochondrial respiratory function.Meanwhile,MT pretreatment reversed the upregulation of P62 induced by PA and activated the AMPK-mTOR-Beclin-1 pathway,contributing to an increase of autophagy and decline apoptosis.Conclusions Our findings indicate that PA can induce mitochondrial dysfunction and enhance autophagy in BEECs.In addition,MT is proved to not only reduce mitochondrial oxidative stress but also facilitate the clearance of dam-aged mitochondria by upregulating autophagy pathways,thereby safeguarding the mitochondrial pool and promot-ing cellular viability.Our study provides a better understanding of the molecular mechanisms underlying the effect of an excess of NEB on the fertility outcomes of high yielding dairy cows.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Epigallocatechin-3-gallate protects bovine ruminal epithelial cells against lipopolysaccharide-induced inflammatory damage by activating autophagy

    Wanli ZhaoTaiyu ShenBichen ZhaoMoli Li...
    2403-2417页
    查看更多>>摘要：Background Subacute ruminal acidosis(SARA)causes an increase in endotoxin,which can induce immune and inflammatory responses in the ruminal epithelium of dairy cows.In non-ruminants,epigallocatechin-3-gallate(EGCG),a major bioactive ingredient of green tea,is well-known to alleviate inflammation.Whether EGCG confers protection against SARA-induced inflammation and the underlying mechanisms are unknown.Results In vivo,eight ruminally cannulated Holstein cows in mid-lactation were randomly assigned to either a low-concentrate(40％)diet(CON)or a high-concentrate(60％)diet(HC)for 3 weeks to induce SARA(n=4).Cows with SARA had greater serum concentrations of tumor necrosis factor(TNF)-α and interleukin-6,and epithelium had histological signs of damage.In vitro,immortalized bovine ruminal epithelial cells(BREC)were treated with lipopoly-saccharide(LPS)to imitate the inflammatory damage caused by SARA.Our data revealed that BREC treated with 10 μg/mL LPS for 6 h successfully induce a robust inflammatory response as indicated by increased phosphoryla-tion of IKBα and nuclear factor kappa-B(NF-κB)p65.Pre-treatment of BREC with 50 μmol/L EGCG for 6 h before LPS challenge promoted the degradation of NLR family pyrin domain containing 3(NLRP3)inflammasome through acti-vation of autophagy,which further repressed activation of NF-κB pathway targeting Toll-like receptor 4(TLR4).Analy-ses also revealed that the ECGG upregulated tight junction(TJ)protein expression upon incubation with LPS.Conclusions Subacute ruminal acidosis causes ruminal epithelium injury and systemic inflammation in dairy cows.However,the anti-inflammatory effects of EGCG help preserve the integrity of the epithelial barrier through activat-ing autophagy when BREC are exposed to LPS.Thus,EGCG could potentially serve as an effective therapeutic agent for SARA-associated inflammation.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    The influence of iron nutrition on the development of intestine and immune cell divergency in neonatal pigs

    Yao LiuAimin WuBing YuJun He...
    2418-2431页
    查看更多>>摘要：Background Appropriate iron supplementation is essential for neonatal growth and development.However,there are few reports on the effects of iron overload on neonatal growth and immune homeostasis.Thus,the aim of this study was to investigate the effects of iron nutrition on neonatal growth and intestinal immunity by administering different levels of iron to neonatal pigs.Results We found that iron deficiency and iron overload resulted in slow growth in neonatal pigs.Iron deficiency and iron overload led to down-regulation of jejunum intestinal barrier and antioxidant marker genes,and promoted CD8+T cell differentiation in jejunum and mesenteric lymph nodes(MLN)of pigs,disrupting intestinal health.Moreo-ver,iron levels altered serum iron and tissue iron status leading to disturbances in redox state,affecting host innate and adaptive immunity.Conclusions These findings emphasized the effect of iron nutrition on host health and elucidated the importance of iron in regulating redox state and immunity development.This study provided valuable insights into the regulation of redox state and immune function by iron metabolism in early life,thus contributing to the development of tar-geted interventions and nutritional strategies to optimize iron nutrition in neonates.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    CAMKK2-AMPK axis endows dietary calcium and phosphorus levels with regulatory effects on lipid metabolism in weaned piglets

    Zhenyan MiaoYanjie SunZhangjian FengQiwen Wu...
    2432-2451页
    查看更多>>摘要：Background In the realm of swine production,optimizing body composition and reducing excessive fat accumula-tion is critical for enhancing both economic efficiency and meat quality.Despite the acknowledged impact of dietary calcium(Ca)and phosphorus(P)on lipid metabolism,the precise mechanisms behind their synergistic effects on fat metabolism remain elusive.Results Research observations have shown a decreasing trend in the percentage of crude fat in carcasses with increased calcium and phosphorus content in feed.Concurrently,serum glucose concentrations significantly decreased,though differences in other lipid metabolism-related indicators were not significant across groups.Under conditions of low calcium and phosphorus,there is a significant suppression in the expression of FABPs,CD36 and PPARy in the jejunum and ileum,leading to inhibited intestinal lipid absorption.Concurrently,this results in a marked increase in lipid accumulation in the liver.Conversely,higher levels of dietary calcium and phosphorus promoted intestinal lipid absorption and reduced liver lipid accumulation,with these changes being facilitated through the activation of the CAMKK2/AMPK signaling pathway by high-calcium-phosphorus diets.Additionally,the levels of calcium and phosphorus in the diet significantly altered the composition of liver lipids and the gut micro-biota,increasing α-diversity and affecting the abundance of specific bacterial families related to lipid metabolism.Conclusion The evidence we provide indicates that the levels of calcium and phosphorus in the diet alter body fat content and lipid metabolism by modulating the response of the gut-liver axis to lipids.These effects are closely asso-ciated with the activation of the CAMKK2/AMPK signaling pathway.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Prebiotic galactooligosaccharide improves piglet growth performance and intestinal health associated with alterations of the hindgut microbiota during the peri-weaning period

    Timothy E.BostonFeng WangXi LinSung Woo Kim...
    2452-2466页
    查看更多>>摘要：Background Weaning stress reduces growth performance and health of young pigs due in part to an abrupt change in diets from highly digestible milk to fibrous plant-based feedstuffs.This study investigated whether dietary galactoo-ligosaccharide(GOS),supplemented both pre-and post-weaning,could improve growth performance and intestinal health via alterations in the hindgut microbial community.Methods Using a 3×2 factorial design,during farrowing 288 piglets from 24 litters received either no creep feed(FC),creep without GOS(FG-)or creep with 5％GOS(FG+)followed by a phase 1 nursery diet without(NG-)or with 3.8％GOS(NG+).Pigs were sampled pre-(D22)and post-weaning(D31)to assess intestinal measures.Results Creep fed pigs grew 19％faster than controls(P＜0.01)prior to weaning,and by the end of the nursery phase(D58),pigs fed GOS pre-farrowing(FG+)were 1.85 kg heavier than controls(P＜0.05).Furthermore,pigs fed GOS in phase 1 of the nursery grew 34％faster(P＜0.04),with greater feed intake and efficiency.Cecal microbial com-munities clustered distinctly in pre-vs.post-weaned pigs,based on principal coordinate analysis(P＜0.01).No effects of GOS were detected pre-weaning,but gruel creep feeding increased Chao1 α-diversity and altered several genera in the cecal microbiota(P＜0.05).Post-weaning,GOS supplementation increased some genera such as Fusicateni-bacterand Collinsella,whereas others decreased such as Campylobacter and Frisingicoccus(P＜0.05).Changes were accompanied by higher molar proportions of butyrate in the cecum of GOS-fed pigs(P＜0.05).Conclusions Gruel creep feeding effectively improves suckling pig growth regardless of GOS treatment.When sup-plemented post-weaning,prebiotic GOS improves piglet growth performance associated with changes in hindgut microbial composition.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Effects and interactions of dietary lysine and apparent nitrogen corrected metabolizable energy on yellow-feathered broiler breeder hens

    Yibing WangQin WangXiangtian YaoZhongyong Gou...
    2467-2482页
    查看更多>>摘要：Background Dietary lysine and apparent nitrogen-corrected metabolizable energy(AMEn)are two key variables affecting the production of breeder hens.In this study,the effects and interactions of lysine and AMEn on yellow-feathered broiler breeder hens were investigated.A total of 720 30-week-old breeder hens were fed in a 5(lysine:0.56％,0.68％,0.80％,0.92％,and 1.04％)×2(AMEn:11.50 and 11.10 MJ/kg)factorial arrangement for 12 weeks.The productive performance,reproductive traits,biochemical variables of breeder hens,the amino acid concentration and quality of eggs,and the growth performance of offspring broilers were determined.Result(1)Dietary lysine had quadratic effects(P＜0.05)on laying rate,average daily egg mass and feed intake/egg mass of breeder hens;birds with 11.50 MJ/kg AMEn(high AMEn)had higher(P＜0.05)BWthan those with 11.10 MJ/kg AMEn(low AMEn);(2)dietary lysine significantly affected on the relative ovarian weight(quadratic and linear),and numbers of large yellow follicles(LYF,quadratic);birds with high AMEn had longer fallopian tube and more LYF than those with low AMEn(P＜0.05);(3)dietary lysine had significant effects(linear and quadratic)on eggshell thickness and shell strength of eggs from breeder hens;birds with high AMEn had thinner eggshells and deeper yolk color than those with low AMEn(P＜0.05);(4)there were higher(P＜0.05)contents of protein and concentrations of all measured animo acids(AAs)in eggs from birds fed low AMEn;(5)supplementation with high AMEn to breeder hens significantly increased the hatchability of fertilized eggs;(6)neither dietary lysine level or AMEn affected growth performance of offspring broilers;(7)both dietary lysine level and AMEn significantly affected gonadotropin concen-trations and biochemical variables of breeder hens.Conclusions Dietary lysine had significant influences on productive performance,reproductive traits,and egg quality of yellow-feathered breeder hens.Based on productive performance,the optimal levels of dietary lysine were 0.81％to 0.83％,while 0.71％to 72％lysine was enough to obtain the best quality of breeding eggs.High AMEn was more beneficial to breeder hens for reproductive traits and hatchability of the fertilized eggs,while it showed detrimental effects on eggshell thickness and AA concentrations of breeding eggs.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens

    Minghui WangKelin LiHongchao JiaoJingpeng Zhao...
    2483-2505页
    查看更多>>摘要：Background High-fat diets(HFD)are known to enhance feed conversion ratio in broiler chickens,yet they can also result in hepatic fat accumulation.Bile acids(BAs)and gut microbiota also play key roles in the formation of fatty liver.In this study,our objective was to elucidate the mechanisms through which BA supplementation reduces hepatic fat deposition in broiler chickens,with a focus on the involvement of gut microbiota and liver BA composition.Results Newly hatched broiler chickens were allocated to either a low-fat diet(LFD)or HFD,supplemented with or without BAs,and subsequently assessed their impacts on gut microbiota,hepatic lipid metabolism,and hepatic BA composition.Our findings showed that BA supplementation significantly reduced plasma and liver tissue triglyceride(TG)levels in 42-day-old broiler chickens(P＜0.05),concurrently with a significant decrease in the expression levels of fatty acid synthase(FAS)in liver tissue(P＜0.05).These results suggest that BA supple-mentation effectively diminishes hepatic fat deposition.Under the LFD,BAs supplementation increased the BA content and ratio of Non 12-OH BAs/12-OH BAs in the liver and increased the Akkermansia abundance in cecum.Under the HFD,BA supplementation decreased the BAs and increased the relative abundances of chenodeoxycholic acid(CDCA)and cholic acid(CA)in hepatic tissue,while the relative abundances of Bacteroides were dramatically reduced and the Bifidobacterium,Escherichia,and Lactobacillus were increased in cecum.Correlation analyses showed a significant positive correlation between the Akkermansia abundance and Non 12-OH BA content under the LFD,and presented a significant negative correlation between the Bacteroides abundance and CA or CDCA content under the HFD.Conclusions The results indicate that supplementation of BAs in both LFD and HFD may ameliorate hepatic fat deposition in broiler chickens with the involvement of differentiated microbiota-bile acid profile pathways.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Probiotic Lactobacillus rhamnosus GG improves insulin sensitivity and offspring survival via modulation of gut microbiota and serum metabolite in a sow model

    Tianle GaoRan LiLiang HuQuanfang Hu...
    2506-2523页
    查看更多>>摘要：Background Sows commonly experience insulin resistance in late gestation and lactation,causing lower feed intake and milk production,which can lead to higher mortality rates in newborn piglets.The probiotic Lactobacillus rham-nosus GG(LGG)is known to improve insulin resistance.However,whether supplementing LGG can improve insulin sensitivity in sows and enhance lactation performance,particularly the early survival of offspring remains unclear.Hence,we explored the effects and mechanisms of supplementing LGG during late gestation and lactation on sow insulin sensitivity,lactation performance,and offspring survival.In total,20 sows were randomly allocated to an LGG(n=10)and control group(n=10).Results In sows,LGG supplementation significantly improved insulin sensitivity during late gestation and lactation,increased feed intake,milk production and colostrum lactose levels in early lactation,and enhanced newborn piglet survival.Moreover,LGG treatment significantly reshaped the gut microbiota in sows,notably increasing microbiota diversity and enriching the relative abundance of insulin sensitivity-associated probiotics such as Lactobacillus,Bifido-bacterium,and Bacteroides.Serum metabolite and amino acid profiling in late-gestation sows also revealed decreased branched-chain amino acid and kynurenine serum levels following LGG supplementation.Further analyses high-lighted a correlation between mitigated insulin resistance in late pregnancy and lactation by LGG and gut microbiota reshaping and changes in serum amino acid metabolism.Furthermore,maternal LGG enhanced immunity in new-born piglets,reduced inflammation,and facilitated the establishment of a gut microbiota.Conclusions We provide the first evidence that LGG mitigates insulin resistance in sows and enhances offspring survival by modulating the gut microbiota and amino acid metabolism.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据