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植物学报（英文版）
植物学报（英文版）

刘春明

月刊

1672-9072

jipb@ibcas.ac.cn

010-62836133，010-62836563

100093

北京香山南辛村20号中科院植物所内

植物学报（英文版）/Journal Journal of Integrative Plant BiologyCSCDCSTPCD北大核心SCI
查看更多>>本学报是植物学综合性学术期刊。国外发行与交换达40多个国家和地区。国际标准A4大16开铜版纸印刷。本学报力争全面反映我国植物科学的最新研究成果，关注国际热点、新的学科生长点、前沿研究课题，重视报道重要的应用基础研究。主要栏目有植物生理生化、植物遗传学和分子生物学、植物生殖生物学、结构植物学、植物化学与资源植物学、植物系统与进化、植物生态学、古植物学的原始研究论文、综述和快讯。
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    Two-faced OsNAS3 influences disease resistance via nicotianamine and ethylene

    Kaiwei HeLiting XuQin HeWei Zhang...
    2581-2585页
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Reading m6A marks in mRNA:A potent mechanism of gene regulation in plants

    Thi Kim Hang NguyenHunseung Kang
    2586-2599页
    查看更多>>摘要：Modifications to RNA have recently been recog-nized as a pivotal regulator of gene expression in living organisms.More than 170 chemical mod-ifications have been identified in RNAs,with N6-methyladenosine(m6A)being the most abundant modification in eukaryotic mRNAs.The addition and removal of m6A marks are catalyzed by meth-yltransferases(referred to as"writers")and deme-thylases(referred to as"erasers"),respectively.In addition,the m6A marks in mRNAs are recognized and interpreted by m6A-binding proteins(referred to as"readers"),which regulate the fate of mRNAs,including stability,splicing,transport,and trans-lation.Therefore,exploring the mechanism under-lying the m6A reader-mediated modulation of RNA metabolism is essential for a much deeper under-standing of the epigenetic role of RNA modification in plants.Recent discoveries have improved our understanding of the functions of m6A readers in plant growth and development,stress response,and disease resistance.This review highlights the latest developments in m6A reader research,em-phasizing the diverse RNA-binding domains crucial for m6A reader function and the biological and cel-lular roles of m6A readers in the plant response to developmental and environmental signals.More-over,we propose and discuss the potential future research directions and challenges in identifying novel m6A readers and elucidating the cellular and mechanistic role of m6A readers in plants.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    HOS1 ubiquitinates SPL9 for degradation to modulate salinity-delayed flowering

    Zhixin JiaoXiaoning ShiRui XuMingxia Zhang...
    2600-2612页
    查看更多>>摘要：Soil salinity is a serious environmental threat to plant growth and flowering.Flowering in the right place,at the right time,ensures maximal re-productive success for plants.Salinity-delayed flowering is considered a stress coping/survival strategy and the molecular mechanisms underlying this process require further studies to enhance the crop's salt tolerance ability.A nuclear pore com-plex(NPC)component,HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 1(HOS1),has been recognized as a negative regulator of plant cold responses and flowering.Here,we challenged the role of HOS1 in regulating flowering in response to salinity stress.Interestingly,we discovered that HOS1 can directly interact with and ubiquitinate transcription factor SPL9(SQUAMOSA PRO-MOTER BINDING PROTEIN-LIKE 9)to promote its protein degradation in response to salinity stress.Moreover,we demonstrated that HOS1 and SPL9 antagonistically regulate plant flowering under both normal and salt stress conditions.HOS1 was fur-ther shown to negatively regulate the expression of SPLs and several key flowering genes in response to salinity stress.These results jointly revealed that HOS1 is an important integrator in the process of modulating salinity-delayed flowering,thus offering new perspectives on a salinity stress coping strategy of plants.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    The METHYLTRANSFERASE B-SERRATE interaction mediates the reciprocal regulation of microRNA biogenesis and RNA m6A modification

    Haiyan BaiYanghuan DaiPanting FanYiming Zhou...
    2613-2631页
    查看更多>>摘要：In eukaryotes,RNA N6-methyladenosine(m6A)modification and microRNA(miRNA)-mediated RNA silencing represent two critical epigenetic regulatory mechanisms.The m6A methyl-transferase complex(MTC)and the micro-processor complex both undergo liquid-liquid phase separation to form nuclear membraneless organelles.Although m6A methyltransferase has been shown to positively regulate miRNA biogenesis,a mechanism of reciprocal regu-lation between the MTC and the microprocessor complex has remained elusive.Here,we demonstrate that the MTC and the micro-processor complex associate with each other through the METHYLTRANSFERASE B(MTB)-SERRATE(SE)interacting module.Knockdown of MTB impaired miRNA biogenesis by diminishing microprocessor complex binding to primary miRNAs(pri-miRNAs)and their re-spective MIRNA loci.Additionally,loss of SE function led to disruptions in transcriptome-wide m6A modification.Further biochemical assays and fluorescence recovery after photobleaching(FRAP)assay indicated that SE enhances the liquid-liquid phase separation and solubility of the MTC.Moreover,the MTC exhibited en-hanced retention on chromatin and diminished binding to its RNA substrates in the se mutant background.Collectively,our results reveal the substantial regulatory interplay between RNA m6A modification and miRNA biogenesis.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    GhCASPL1 regulates secondary cell wall thickening in cotton fibers by stabilizing the cellulose synthase complex on the plasma membrane

    Li ZhangXingpeng WenXin ChenYifan Zhou...
    2632-2647页
    查看更多>>摘要：Cotton(Gossypium hirsutum)fibers are elongated single cells that rapidly accumulate cellulose during secondary cell wall(SCW)thickening,which requires cellulose synthase complex(CSC)activity.Here,we describe the CSC-interacting factor CASPARIAN STRIP MEMBRANE DOMAIN-LIKE1(GhCASPL1),which contributes to SCW thickening by influencing CSC stability on the plasma membrane.GhCASPL1 is preferentially expressed in fiber cells during SCW biosynthesis and encodes a MARVEL domain protein.The ghcaspl1 ghcaspl2 mutant exhibited reduced plant height and produced mature fibers with fewer natural twists,lower tensile strength,and a thinner SCW compared to the wild type.Similarly,the Arabidopsis(Arabidopsis thaliana)caspl1 caspl2 double mutant showed a lower cellulose content and thinner cell walls in the stem vasculature than the wild type but normal plant morphology.Introducing the cotton gene GhCASPL1 successfully restored the reduced cellulose content of the Arabidopsis caspl1 caspl2 mutant.Detergent treatments,ultra-centrifugation assays,and enzymatic assays showed that the CSC in the ghcaspl1 ghcaspl2 double mutant showed reduced membrane binding and decreased enzyme activity compared to the wild type.GhCASPL1 binds strongly to phosphatidic acid(PA),which is present in much higher amounts in thickening fiber cells compared to ovules and leaves.Mutating the PA-binding site in GhCASPL1 resulted in the loss of its colocalization with GhCesA8,and it failed to localize to the plasma membrane.PA may alter membrane structure to facilitate protein-protein interactions,suggesting that GhCASPL1 and PA collaboratively stabilize the CSC.Our findings shed light on CASPL functions and the molecular machi-nery behind SCW biosynthesis in cotton fibers.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    The OsMAPK5-OsWRKY72 module negatively regulates grain length and grain weight in rice

    Fuxiang WangJiexin LinFan YangXiaofeng Chen...
    2648-2663页
    查看更多>>摘要：Grain size and grain weight are important de-terminants for grain yield.In this study,we identify a novel OsMAPK5-OsWRKY72 module that negatively regulates grain length and grain weight in rice.We found that loss-of-function of OsMAPK5 leads to larger cell size of the rice spikelet hulls and a significant increase in both grain length and grain weight in an indica variety Minghui 86(MH86).OsMAPK5 interacts with OsMAPKK3/4/5 and OsWRKY72 and phosphor-ylates OsWRKY72 at T86 and S88.Similar to the osmapk5 MH86 mutants,the oswrky72 knockout MH86 mutants exhibited larger size of spikelet hull cells and increased grain length and grain weight,whereas the OsWRKY72-overexpression MH86 plants showed opposite phenotypes.OsWRKY72 targets the W-box motifs in the promoter of OsARF6,an auxin response factor involved in auxin signaling.Dual-luciferase re-porter assays demonstrated that OsWRKY72 activates OsARF6 expression.The activation effect of the phosphorylation-mimicking OsWR-KY72T86D/S88D on OsARF6 expression was sig-nificantly enhanced,whereas the effects of the OsWRKY72 phosphorylation-null mutants were significantly reduced.In addition,auxin levels in young panicles of the osmapk5 and oswrky72 mutants were significantly higher than that in the wild-type MH86.Collectively,our study un-covered novel connections of the OsMAPKK 3/4/5-OsMAPK5-mediated MAPK signaling,OsWRKY72-mediated transcription regulation,and OsARF6-mediated auxin signaling pathways in regulating grain length and grain weight in an indica-type rice,providing promising targets for molecular breeding of rice varieties with high yield and quality.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Phylotranscriptomic and ecological analyses reveal the evolution and morphological adaptation of Abies

    Zhou-Rui WeiDan JiaoChristian Anton WehenkelXiao-Xin Wei...
    2664-2682页
    查看更多>>摘要：Coniferous forests are under severe threat of the rapid anthropogenic climate warming.Abies(firs),the fourth-largest conifer genus,is a keystone component of the boreal and temperate dark-coniferous forests and harbors a remarkably large number of relict taxa.However,the uncertainty of the phylogenetic and biogeographic history of Abies significantly impedes our prediction of future dy-namics and efficient conservation of firs.In this study,using 1,533 nuclear genes generated from transcriptome sequencing and a complete sampling of all widely recognized species,we have success-fully reconstructed a robust phylogeny of global firs,in which four clades are strongly supported and all intersectional relationships are resolved,although phylogenetic discordance caused mainly by in-complete lineage sorting and hybridization was detected.Molecular dating and ancestral area reconstruction suggest a Northern Hemisphere high-latitude origin of Abies during the Late Creta-ceous,but all extant firs diversified during the Mio-cene to the Pleistocene,and multiple continental and intercontinental dispersals took place in re-sponse to the late Neogene climate cooling and orogenic movements.Notably,four critically en-dangered firs endemic to subtropical mountains of China,including A.beshanzuensis,A.ziyuanensis,A.fanjingshanensis and A.yuanbaoshanensis from east to west,have different origins and evolutionary histories.Moreover,three hotspots of species rich-ness,including western North America,central Japan,and the Hengduan Mountains,were identi-fied in Abies.Elevation and precipitation,particularly precipitation of the coldest quarter,are the most significant environmental factors driving the global distribution pattern of fir species diversity.Some morphological traits are evolutionarily constrained,and those linked to elevational variation(e.g.,purple cone)and cold resistance(e.g.,pubescent branch and resinous bud)may have contributed to the di-versification of global firs.Our study sheds new light on the spatiotemporal evolution of global firs,which will be of great help to forest management and species conservation in a warming world.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Sucrose induces flowering by degradation of the floral repressor Ghd7 via K48-linked polyubiquitination in rice

    Lae-Hyeon ChoJinmi YoonGibeom BaekWin Tun...
    2683-2700页
    查看更多>>摘要：Sucrose functions as a signaling molecule in several metabolic pathways as well as in various devel-opmental processes.However,the molecular mech-anisms by which sucrose regulates these processes remain largely unknown.In the present study,we demonstrate that sucrose promotes flowering by mediating the stability of a regulatory protein that represses flowering in rice.Exogenous application of sucrose promoted flowering by inducing florigen gene expression.Reduction of sucrose levels in the phloem through genetic modifications,such as the overexpression of the vacuolar invertase OsVIN2 or the mutation of OsSUT2,a sucrose transporter,de-layed flowering.Analysis of relative transcript levels of floral regulatory genes showed that sucrose acti-vated Ehd1 upstream of the florigen,with no sig-nificant effect on the expression of other upstream genes.Examination of protein stability after sucrose treatment of major floral repressors revealed that the Ghd7 protein was specifically degraded.The Ghd7 protein interacted with the E3 ligase IPA INTER-ACTING PROTEIN1(IPI1),and sucrose-induced K48-linked polyubiquitination of Ghd7 via IPI1,leading to protein degradation.Mutants defective in IPI1 de-layed flowering,confirming its role in modulating proteins involved in flowering.We conclude that su-crose acts as a signaling molecule to induce flow-ering by promoting Ghd7 degradation via IPI1.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    The miR396a-SIGRF8 module regulates sugar accumulation in the roots via SISTP10 during the interaction between root-knot nematodes and tomato plants

    Lulu SunMengting ZhuXiaoxuan ZhouRuiyue Gu...
    2701-2715页
    查看更多>>摘要：Root-knot nematodes(RKNs;Meloidogyne spp.)are a serious threat to crop production.The competition between plants and pathogens for assimilates influences the outcome of their in-teractions.However,the mechanisms by which plants and nematodes compete with each other for assimilates have not been elucidated.In this study,we demonstrated that miR396a plays a negative role in defense against RKNs and a positive role in sugar accumulation in tomato roots.The overexpression of SIGRF8(Solanum lycopersicum growth-regulating factor 8),the target of miR396a,decreased the sugar content of the roots and the susceptibility to RKNs,whereas the grf8-cr mutation had the opposite effects.Furthermore,we confirmed that SIGRF8 regulated the sugar content in roots by directly activating the transcription of SISTP10(Sol-anum lycopersicum sugar transporter protein 10)in response to RKN stress.Moreover,SISTP10 was expressed primarily in the tissues surrounding giant cells,and the SISTP10 knockout increased both the sugar content in the roots and the plant's susceptibility to RKNs.Overall,this study provides important insight into the molecular mechanism through which the miR396a-SIGRF8-SISTP10 module regulates sugar allocation in roots under RKN stress.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Haplotype-resolved genome of a heterozygous wild peach reveals the PdaWRKY4-PdaCYP716A1 module mediates resistance to aphids by regulating betulin biosynthesis

    Jun-Xiu WangYong LiXin-Wei WangKe Cao...
    2716-2735页
    查看更多>>摘要：Wild species of domesticated crops provide valuable genetic resources for resistance breeding.Prunus davidiana,a wild relative of peach with high heterozygosity and diverse stress tolerance,exhibits high resistance against aphids.However,the highly hetero-zygous genome of P.davidiana makes de-termining the underlying factors influencing re-sistance traits challenging.Here,we present the 501.7 Mb haplotype-resolved genome as-sembly of P.davidiana.Genomic comparisons of the two haplotypes revealed 18,152 struc-tural variations,2,699 Pda_hap1-specific and 2,702 Pda_hap2-specific genes,and 1,118 allele-specific expressed genes.Genome com-position indicated 4.1％of the P.davidiana genome was non-peach origin,out of which 94.5％was derived from almond.Based on the haplotype genome,the aphid resistance quan-titative trait locus(QTL)was mapped at the end of Pda03.From the aphid resistance QTL,PdaWRKY4 was identified as the major domi-nant gene,with a 9-bp deletion in its promoter of the resistant phenotype.Specifically,PdaWRKY4 regulates aphid resistance by pro-moting PdaCYP716A1-mediated anti-aphid metabolite betulin biosynthesis.Moreover,we employed a genome design to develop a breeding workflow for rapidly and precisely producing aphid-resistant peaches.In con-clusion,this study identifies a novel aphid re-sistance gene and provides insights into ge-nome design for the development of resistant fruit cultivars.
      原文链接:
    • NETL
    • NSTL
    • 万方数据