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动物学研究
中国科学院昆明动物研究所 中国动物学会
动物学研究

中国科学院昆明动物研究所 中国动物学会

姚永刚

双月刊

0254-5853

zoores@mail.kiz.ac.cn

0871-65199026

650223

昆明市教场东路32号中国科学院昆明动物研究所

动物学研究/Journal Zoological ResearchCSCD北大核心CSTPCDSCI
查看更多>>本刊创刊于1980年。是中国科学院昆明动物研究所主办的向国内外公开发行的学报级学术性期刊。以报道我国动物学领域的新成果、新进展为已任。在《中文核心期刊要目总览》中多次被列为动物学类核心期刊。本刊辟有研究论文、综述、简报、通讯、书评等栏目，主要刊登动物学领域各分支学科有创新性的基础和应用基础研究报告；结合本人研究工作，反映国际最新研究水平的综述；研究简报、快报；新书评介等。近年发表论文中，动物生态医学、进化生物学分别占30%以上。本刊读者对象为科研机构、大专院校从事动物学研究、教学以及资源环境保护与管理的有关人员；也可为从事生命科学、医学、农林牧渔等方面的科研、教学和生产管理人员提供参考资料。
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    Effects of gradient high-field static magnetic fields on diabetic mice

    Biao YuChao SongChuan-Lin FengJing Zhang...
    249-258页
    查看更多>>摘要：Although 9.4 T magnetic resonance imaging(MRI)has been tested in healthy volunteers,its safety in diabetic patients is unclear.Furthermore,the effects of high static magnetic fields(SMFs),especially gradient vs.uniform fields,have not been investigated in diabetics.Here,we investigated the consequences of exposure to 1.0-9.4 T high SMFs of different gradients(＞10 T/m vs.0-10 T/m)on type 1 diabetic(T1D)and type 2 diabetic(T2D)mice.We found that 14 h of prolonged treatment of gradient(as high as 55.5 T/m)high SMFs(1.0-8.6 T)had negative effects on T1D and T2D mice,including spleen,hepatic,and renal tissue impairment and elevated glycosylated serum protein,blood glucose,inflammation,and anxiety,while 9.4 T quasi-uniform SMFs at 0-10 T/m did not induce the same effects.In regular T1D mice(blood glucose ≥16.7 mmol/L),the＞10 T/m gradient high SMFs increased malondialdehyde(P＜0.01)and decreased superoxide dismutase(P＜0.05).However,in the severe T1D mice(blood glucose ≥30.0 mmol/L),the＞10 T/m gradient high SMFs significantly increased tissue damage and reduced survival rate.In vitro cellular studies showed that gradient high SMFs increased cellular reactive oxygen species and apoptosis and reduced MS-1 cell number and proliferation.Therefore,this study showed that prolonged exposure to high-field(1.0-8.6 T)＞10 T/m gradient SMFs(35-1 380 times higher than that of current clinical MRI)can have negative effects on diabetic mice,especially mice with severe T1D,whereas 9.4 T high SMFs at 0-10 T/m did not produce the same effects,providing important information for the future development and clinical application of SMFs,especially high-field MRI.
      原文链接:
    • 万方数据
    • 维普

    Transfer of the zp3a gene results in changes in egg adhesiveness and buoyancy in transgenic zebrafish

    Yu-Qing CaoYong-Xin WangYan ZhaoJia Zhang...
    259-268页
    查看更多>>摘要：Reproductive strategies and spawning habits play key roles in the evolution of endemic East Asian cyprinids.However,the molecular mechanisms underlying the regulation of spawning habits are not well understood.We recently identified zona pellucida(Zp)as the top differentially expressed protein between East Asian cyprinids that produce adhesive and semi-buoyant eggs,suggesting that Zp protein may play important roles in the regulation of egg type.In this work,we generated transgenic zebrafish in which oocyte-specific expression of zp genes from rare minnow(Gobiocypris rarus),an East Asian cyprinid laying adhesive eggs,was driven by a zebrafish zp3.2 gene promoter.We found that the transgenic eggs obtained partial adhesiveness and exhibited alteration in hydration and buoyancy.Abnormal metabolism of vitellogenin(VTG)may contribute to enhanced hydration and/or buoyancy.Our work shows that expression of the exogenous zp3a gene from an adhesive-egg producing fish is sufficient to induce changes in both egg adhesiveness and buoyancy in zebrafish,emphasizing the important role of zp genes in the regulation of spawning habits.Our results thus provide new insights into how endemic East Asian cyprinids may have adapted to the Yangtze river-lake system via changes in spawning habits.
      原文链接:
    • 万方数据
    • 维普

    Single-cell RNA sequencing reveals gonadal dynamic changes during sex differentiation in hermaphroditic protogynous orange-spotted grouper(Epinephelus coioides)

    Xi WuChao-Yue ZhongYang YangTong Wang...
    269-272页
      原文链接:
    • 万方数据
    • 维普

    Complicated expansion trajectories of insertion sequences and potential association with horizontal transfer of Wolbachia DNA

    Yun-Heng MiaoDa-Wei HuangJin-Hua Xiao
    273-275页
      原文链接:
    • 万方数据
    • 维普

    Intercross population study reveals that co-mutation of mitfa genes in two subgenomes induces red skin color in common carp(Cyprinus carpio wuyuanensis)

    Bi-Jun LiLin ChenMeng-Zhen YanXiao-Qing Zou...
    276-286页
    查看更多>>摘要：Common carp are among the oldest domesticated fish in the world.As such,there are many food and ornamental carp strains with abundant phenotypic variations due to natural and artificial selection.Hebao red carp(HB,Cyprinus carpio wuyuanensis),an indigenous strain in China,is renowned for its unique body morphology and reddish skin.To reveal the genetic basis underlying the distinct skin color of HB,we constructed an improved high-fidelity(HiFi)HB genome with good contiguity,completeness,and correctness.Genome structure comparison was conducted between HB and a representative wild strain,Yellow River carp(YR,C.carpio haematopterus),to identify structural variants and genes under positive selection.Signatures of artificial selection during domestication were identified in HB and YR populations,while phenotype mapping was performed in a segregating population generated by HBxYR crosses.Body color in HB was associated with regions with fixed mutations.The simultaneous mutation and superposition of a pair of homologous genes(mitfa)in chromosomes A06 and B06 conferred the reddish color in domesticated HB.Transcriptome analysis of common carp with different alleles of the mitfa mutation confirmed that gene duplication can buffer the deleterious effects of mutation in allotetraploids.This study provides new insights into genotype-phenotype associations in allotetraploid species and lays a foundation for future breeding of common carp.
      原文链接:
    • 万方数据
    • 维普

    Long-read genome assemblies reveal a cis-regulatory landscape associated with phenotypic divergence in two sister Siniperca fish species

    Guang-Xian TuXin-Shuang ZhangRui-Run JiangLong Zhang...
    287-302页
    查看更多>>摘要：Due to the difficulty in accurately identifying structural variants(SVs)across genomes,their impact on cis-regulatory divergence of closely related species,especially fish,remains to be explored.Recently identified broad H3K4me3 domains are essential for the regulation of genes involved in several biologica-processes.However,the role of broad H3K4me3 domains in phenotypic divergence remains poorly understood.Siniperca chuatsi and S.scherzeri are closely related but divergent in severa-phenotypic traits,making them an ideal model to study cis-regulatory evo-ution in sister species.Here,we generated chromosome-leve-genomes of S.chuatsi and S.scherzeri,with assembled genome sizes of 716.35 and 740.54 Mb,respectively.The evo-utionary histories of S.chuatsi and S.scherzeri were studied by inferring dynamic changes in ancestra-population sizes.To explore the genetic basis of adaptation in S.chuatsi and S.scherzeri,we performed gene family expansion and contraction analysis and identified positively se-ected genes(PSGs).To investigate the role of SVs in cis-regulatory divergence of closely re-ated fish species,we identified high-quality SVs as well as divergent H3K27ac and H3K4me3 domains in the genomes of S.chuatsi and S.scherzeri.Integrated analysis revealed that cis-regulatory divergence caused by SVs played an essential role in phenotypic divergence between S.chuatsi and S.scherzeri.Additionally,divergent broad H3K4me3 domains were mostly associated with cancer-related genes in S.chuatsi and S.scherzeri and contributed to their phenotypic divergence.
      原文链接:
    • 万方数据
    • 维普

    A 1.1 Mb duplication CNV on chromosome 17 contributes to skeletal muscle development in Boer goats

    Ying YuanWei-Yi ZhangBai-Gao YangDong-Ke Zhou...
    303-314页
    查看更多>>摘要：The Boer goat is one of the top meat breeds in modern animal husbandry and has attracted widespread attention for its unique growth performance.However,the genetic basis of muscle development in the Boer goat remains obscure.In this study,we identified specific structural variants in the Boer goat based on genome-wide selection signals and analyzed the basis of the molecular heredity of related candidate genes in muscle development.A total of 9 959 autosomal copy number variations(CNVs)were identified through selection signal analysis in 127 goat genomes.Specifically,we confirmed that the highest signal CNV(HSV)was a chromosomal arrangement containing an approximately 1.11 Mb(CHIR17:60062304-61171840 bp)duplicated fragment inserted in reverse orientation and a 5 362 bp deleted region(CHIR17:60145940-60151302 bp)with overlapping genes(e.g.,ARHGAP10,NR3C2,EDNRA,PRMT9,and TMEM184C).The homozygous duplicated HSV genotype(+/+)was found in 96％of Boer goats but was not detected in Eurasian goats and was only detected in 4％of indigenous African goats.The expression network of three candidate genes(ARHGAP10,NR3C2,and EDNRA)regulating dose transcription was constructed by RNA sequencing.Results indicated that these genes were involved in the proliferation and differentiation of skeletal muscle satellite cells(SMSCs)and their overexpression significantly increased the expression of SAA3.The HSV of the Boer goat contributed to superior skeletal muscle growth via the dose effects of overlapping genes.
      原文链接:
    • 万方数据
    • 维普

    Adult neurogenesis in the primate hippocampus

    Ye LiNa-Na XuZhao-Zhe HaoSheng Liu...
    315-322页
    查看更多>>摘要：Adult hippocampal neurogenesis(AHN)is crucial for learning,memory,and emotion.Deficits of AHN may lead to reduced cognitive abilities and neurodegenerative disorders,such as Alzheimer's disease.Extensive studies on rodent AHN have clarified the developmental and maturation processes of adult neural stem/progenitor cells.However,to what extent these findings apply to primates remains controversial.Recent advances in next-generation sequencing technologies have enabled in-depth investigation of the transcriptome of AHN-related populations at single-cell resolution.Here,we summarize studies of AHN in primates.Results suggest that neurogenesis is largely shared across species,but substantial differences also exist.Marker gene expression patterns in primates differ from those of rodents.Compared with rodents,the primate hippocampus has a higher proportion of immature dentate granule cells and a longer maturation period of newly generated granule cells.Future research on species divergence may deepen our understanding of the mechanisms underlying adult neurogenesis in primates.
      原文链接:
    • 万方数据
    • 维普

    Ferrets:A powerful model of SARS-CoV-2

    Yan ZhaoChang-Le WangZhi-Yun GaoHong-Xiu Qiao...
    323-330页
    查看更多>>摘要：The rapid spread of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)in recent years not only caused a global pandemic but resulted in enormous social,economic,and health burdens worldwide.Despite considerable efforts to combat coronavirus disease 2019(COVID-19),various SARS-CoV-2 variants have emerged,and their underlying mechanisms of pathogenicity remain largely unknown.Furthermore,effective therapeutic drugs are still under development.Thus,an ideal animal model is crucial for studying the pathogenesis of COVID-19 and for the preclinical evaluation of vaccines and antivirals against SARS-CoV-2 and variant infections.Currently,several animal models,including mice,hamsters,ferrets,and non-human primates(NHPs),have been established to study COVID-19.Among them,ferrets are naturally susceptible to SARS-CoV-2 infection and are considered suitable for COVID-19 study.Here,we summarize recent developments and application of SARS-CoV-2 ferret models in studies on pathogenesis,therapeutic agents,and vaccines,and provide a perspective on the role of these models in preventing COVID-19 spread.
      原文链接:
    • 万方数据

    C9orf72 poly-GA proteins impair neuromuscular transmission

    Wen-Yo TuWentao XuJianmin ZhangShuyuan Qi...
    331-340页
    查看更多>>摘要：Amyotrophic lateral sclerosis(ALS)is a devastating motoneuron disease,in which lower motoneurons lose control of skeletal muscles.Degeneration of neuromuscular junctions(NMJs)occurs at the initial stage of ALS.Dipeptide repeat proteins(DPRs)from G4C2 repeat-associated non-ATG(RAN)translation are known to cause C9orf72-associated ALS(C9-ALS).However,DPR inclusion burdens are weakly correlated with neurodegenerative areas in C9-ALS patients,indicating that DPRs may exert cell non-autonomous effects,in addition to the known intracellular pathological mechanisms.Here,we report that poly-GA,the most abundant form of DPR in C9-ALS,is released from cells.Local administration of poly-GA proteins in peripheral synaptic regions causes muscle weakness and impaired neuromuscular transmission in vivo.The NMJ structure cannot be maintained,as evidenced by the fragmentation of postsynaptic acetylcholine receptor(AChR)clusters and distortion of presynaptic nerve terminals.Mechanistic study demonstrated that extracellular poly-GA sequesters soluble Agrin ligands and inhibits Agrin-MuSK signaling.Our findings provide a novel cell non-autonomous mechanism by which poly-GA impairs NMJs in C9-ALS.Thus,targeting NMJs could be an early therapeutic intervention for C9-ALS.
      原文链接:
    • 万方数据
    • 维普